By W. F. Carson IV, S. L. Kunkel (auth.), Prof. Jean-Louis Vincent (eds.)
The Yearbook compiles the newest advancements in experimental and medical examine and perform in a single finished reference publication. The chapters are written through good well-known specialists within the box of in depth care and emergency medication. it truly is addressed to each person concerned with inner medication, anesthesia, surgical procedure, pediatrics, in depth care and emergency medicine.
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Extra resources for Annual Update in Intensive Care and Emergency Medicine 2012
Various studies have focused chiefly on the administration of IFN-γ  and CSF growth factors (particularly GM-CSF in comparison with G-CSF, which has been seldom studied). The beneficial effects of IFN-γ treatment were originally proposed in ICU patients at the end of the 1980s ; the patients exhibited significant increases in mHLADR expression in vitro after treatment. Later studies did not demonstrate a clini- 24 A. Gouel, A. Lepape, and B. Allaouchiche I Fig. 4. Reversal rate from infection of G-CSF or GM-CSF therapy versus placebo.
Blood samples are collected in EDTA-anticoagulated tubes (ethylenediaminetetraacetic acid). They must be kept at 4 °C and stained within 2 hours after collection, as mHLA-DR is a very sensitive marker and its expression is rapidly regulated in vitro. Flow cytometric analysis can be performed using various techniques during the subsequent 24 hours – if the tubes are kept at 4 °C – without causing variation in the results. The procedure consists of an initial double labeling of the cells with two antibodies: Anti-CD14 – a specific marker for monocytes – and anti-HLA-DR, usually coupled with phycoerythrin.
Venet F, Chung CS, Kherouf H, et al (2009) Increased circulating regulatory T cells (CD4(+)CD25 (+)CD127 (-)) contribute to lymphocyte anergy in septic shock patients. Intensive Care Med 35: 678 – 686 28. Heidecke CD, Hensler T, Weighardt H, et al (1999) Selective defects of T lymphocyte function in patients with lethal intraabdominal infection. Am J Surg 178: 288 – 292 29. Oberholzer C, Oberholzer A, Clare-Salzler M, Moldawer LL (2001) Apoptosis in sepsis: a new target for therapeutic exploration.